Differential gene expression analysis of MAT1-1 and MAT1-2 strains in Aspergillus oryzae

ABSTRACT: The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the RIB40 reference strain. We now report the existence of a complimentary MAT1-2 gene and sequencing of an idiomorph region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of MAT1-1 and MAT1-2 genotype among 180 strains assayed including industrial tane-koji isolates. Strains used for sake and miso production showed a near 1:1 ratio of MAT1-1 and MAT1-2 mating-types, whereas strains used for soy sauce production showed a significant bias towards the MAT1-2 mating type. MAT1-1 and MAT1-2 isogenic strains were then created by genetic manipulatio

ORGANISM(S): Aspergillus oryzae

SUBMITTER: Ryuta Wada 

PROVIDER: E-GEOD-33542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress