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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Disrupted transcripitonal network in ΔNp63 AEC tissue model

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE33495: Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression] GSE33571: Disrupted transcriptional network in ΔNp63 AEC tissue model [ChIP-Seq] Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Kun Qu

PROVIDER: E-GEOD-33572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Disrupted transcriptional network in ΔNp63 AEC tissue model [ChIP-Seq]

Project description:The transcriptional basis for disrupted epidermal differentiation arising from TP63 AEC mutations remains to be elucidated. Here we present an organotypic model of AEC dysfunction that phenocopies differentiation defects observed in AEC patient skin. Transcriptional analysis of model AEC tissue revealed impaired induction of differentiation regulators, including OVOL1, GRHL3, KLF4, PRDM1 and ZNF750. Genome wide binding analyses of TP63 during epidermal differentiation showed direct binding of OVOL1, GRHL3, and ZNF750 promoters suggesting AEC mutants prevent normal activation of these targets by direct transcriptional interference. Remarkably, exogenous ZNF750 restores impaired epidermal differentiation caused by AEC mutation. Thus, repression of ZNF750 is central to disrupted epidermal differentiation in model AEC tissue. ChIP-Seq analysis: Examination of p63 binding in proliferating and differentiating human keratinocytes

2012-06-18 | E-GEOD-33571 | biostudies-arrayexpress

Disrupted transcripitonal network in ΔNp63 AEC tissue model

Project description:Disrupted transcripitonal network in ΔNp63 AEC tissue model

| PRJNA148567 | ENA

Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]

Project description:The transcriptional basis for disrupted epidermal differentiation arising from TP63 AEC mutations remains to be elucidated. Here we present an organotypic model of AEC dysfunction that phenocopies differentiation defects observed in AEC patient skin. Transcriptional analysis of model AEC tissue revealed impaired induction of differentiation regulators, including OVOL1, GRHL3, KLF4, PRDM1 and ZNF750. Genome wide binding analyses of TP63 during epidermal differentiation showed direct binding of OVOL1, GRHL3, and ZNF750 promoters suggesting AEC mutants prevent normal activation of these targets by direct transcriptional interference. Remarkably, exogenous ZNF750 restores impaired epidermal differentiation caused by AEC mutation. Thus, repression of ZNF750 is central to disrupted epidermal differentiation in model AEC tissue. Gene expression analysis: To establish a differentiation signature for primary human keratinocytes, with p63i-depleted, and ΔNp63α AEC mutants overexpressed, total RNA was isolated in biologic duplicate from cells in different conditions and hybridized to Affymetrix HG-U133 2.0 Plus arrays.

2012-06-17 | E-GEOD-33495 | biostudies-arrayexpress

Disrupted transcriptional network in ΔNp63 AEC tissue model [ChIP-Seq]

2012-06-18 | GSE33571 | GEO

Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]

2012-06-18 | GSE33495 | GEO

Homo sapiens

Project description:Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]

| PRJNA154281 | ENA

Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

Project description:A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.

2018-08-29 | GSE119123 | GEO

Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

Project description:Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

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AEC II cell differentiation in miR142 overexpressing mice

Project description:We observed that loss of miR142 leads to the increased expression of surfactant protein C and decreased Podoplanin. This indicates increased differentiation of alveolar progenitors towards AEC II lineage. In order to understand what are the genes that are being regulated during AEC II cell differentiation in miR142 overexpressing mice we are performing this experiment.

2019-11-01 | GSE106410 | GEO

AEC II cell differentiation in miR142 KO mice

Project description:We observed that loss of miR142 leads to the increased expression of surfactant protein C and decreased Podoplanin.This indicates increased differentiation of alveolar progenitors towards AEC II lineage. In order to understand 1)what are the genes that are being regulated during AEC II cell differentiation in miR142KO mice we are performing this experiment.

2019-11-01 | GSE106244 | GEO

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