Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Sugarcane Expression Data from Stress Time Series


ABSTRACT: Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland's solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response RNA from experimental sample and from its respective control sample (untreated plants) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental point. The quantification of each hybridization was submitted in two files, one for each slide side (technical replicates).

ORGANISM(S): Saccharum hybrid cultivar (mixed)

SUBMITTER: Milton Nishiyama Jr 

PROVIDER: E-GEOD-33574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of sense and antisense transcripts regulated by drought in sugarcane.

Lembke Carolina Gimiliani CG   Nishiyama Milton Yutaka MY   Sato Paloma Mieko PM   de Andrade Rodrigo Fandiño RF   Souza Glaucia Mendes GM  

Plant molecular biology 20120519 4-5


Sugarcane is an important sugar and energy crop that can be used efficiently for biofuels production. The development of sugarcane cultivars tolerant to drought could allow for the expansion of plantations to sub-prime regions. Knowledge on the mechanisms underlying drought responses and its relationship with carbon partition would greatly help to define routes to increase yield. In this work we studied sugarcane responses to drought using a custom designed oligonucleotide array with 21,901 diff  ...[more]

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