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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The downstream gene expression of AnxA4 in AGS cells

ABSTRACT: AnxA4 expression is increased in H. pylori associated tumors. Moreover, in renal-cell cancer, AnxA4 increases tumor cell dissemination and promotes cell migration, and in colorectal cancer, it is identified as a potential diagnostic marker. How AnxA4 might be involved in tumorgenesis has remained unclear. Here, we compared the gene expression profiles between the cells transfected with pcDNA3.1 (+)/AnxA4 and control (empty vector) to study the downstream genes of AnxA4 using Exon array technique. Four samples are analyzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Hsueh-Fen Juan

PROVIDER: E-GEOD-33620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Revealing the molecular mechanism of gastric cancer marker annexin A4 in cancer cell proliferation using exon arrays.

Lin Li-Ling LL Huang Hsuan-Cheng HC Juan Hsueh-Fen HF

PloS one 20120907 9

Gastric cancer is a malignant disease that arises from the gastric epithelium. A potential biomarker for gastric cancer is the protein annexin A4 (ANXA4), an intracellular Ca(2+) sensor. ANXA4 is primarily found in epithelial cells, and is known to be involved in various biological processes, including apoptosis, cell cycling and anticoagulation. In respect to cancer, ANXA4-overexpression has been observed in cancers of various origins, including gastric tumors associated with Helicobacter pylor ...[more]

PMID: 22970268

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The downstream gene expression of AnxA4 in AGS cells

Project description:AnxA4 expression is increased in H. pylori associated tumors. Moreover, in renal-cell cancer, AnxA4 increases tumor cell dissemination and promotes cell migration, and in colorectal cancer, it is identified as a potential diagnostic marker. How AnxA4 might be involved in tumorgenesis has remained unclear. Here, we compared the gene expression profiles between the cells transfected with pcDNA3.1 (+)/AnxA4 and control (empty vector) to study the downstream genes of AnxA4 using Exon array technique.

2012-12-20 | GSE33620 | GEO

miRNAs were significantly regulated by myocardin and ERM-NM-1

Project description:Using microarray, we compared miRNAs expression profiles of MDA-MB-231 cells transfected with myocardin and empty vector (pcDNA3.1) and found that 25 miRNAs were significantly changed in myocardin-transfected groups (17 up-regulated and 9 down-regulated miRNAs). Moreover, we showed that 18 of 25 miRNAs significantly regulated by myocardin were inhibited by ERM-NM-1 in MDA-MB-231 cells. In addition,through a microarray approach, we identify the subset of miRNAs modulated by ERM-NM-1 in MDA-MB-231 cells. Our results determined that ERM-NM-1 may function as tumor-promoter through down-regulating expression of 3 miRNAs (miR-26b, miR-146a and miR-331-3p) in MDA-MB-231 cells. There are 4 samples in this experiment (pcDNA3.1-transfected, myocardin-transfected, ERM-NM-1-transfected, myocardin plus ERM-NM-1 co-transfecteded groups) and each sample was replicated 3 times. The vector-pcDNA3.1 was used as control.

2014-10-01 | E-GEOD-44139 | biostudies-arrayexpress

Expression analysis of Human esophagus cancer cell line TE-1 transfected with PCDNA3.1 and PCDNA3.1-Pax2

Project description:Investigation of whole genome gene expression level changes of TE-1 transfected with PCDNA3.1-Pax2 ,compared to TE-1 transfected with PCDNA3.1. Compare PAX2 activated genes by analyzing mRNA profilings between pcDNA3.1 and pcDNA3.1-PAX2- transfected TE-1 cells.

2014-12-12 | E-GEOD-64093 | biostudies-arrayexpress

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AGS-RACK1

Project description:MicroRNA microarray profiling analysis was performed on human gastric cancer AGS cells after RACK1 silenced or not

2016-03-01 | E-MTAB-2619 | biostudies-arrayexpress

Microrray expression data of Osteoarthritis synovial fibroblasts (OASF) transfected with TBX5

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Gene expression regulated by HBV mRNAs

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2014-12-12 | E-GEOD-64084 | biostudies-arrayexpress

mRNA and lncRNA expression profiles in MCF-7 breast cancer cells stably overexpressing pcDNA3.1-PinX1 and Empty Vector

Project description:To elucidate the molecular mechanism and signaling pathways that PinX1 is involved in, a Human LncRNA Array V2.0 (Arraystar, USA)-based genome wide screen of the alterations in lncRNA and mRNA expression profiles was performed in MCF-7 cells stably overexpressing PinX1. MCF-7 breast cancer cells were transfected with the pcDNA3.1-PinX1 and pcDNA3.1 empty vector and the microarray analysis were performed after the clonal selection of cells with a high expression level.

2013-09-30 | E-GEOD-46756 | biostudies-arrayexpress

L1CAM overexpression in mouse lung endothelial cells (lECs)

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2014-10-21 | E-GEOD-45859 | biostudies-arrayexpress

Genome-wide analysis of MCF-7 cells expressing or not MMP-14 (MT1-MMP) growing in monolayer cell culture conditions

Project description:In order to investigate the impact of MMP-14 (MT1-MMP) on the transcriptomes of a human breast adenocarcinoma cell line, we performed a microarray analysis from RNAs isolated from MCF-7 cells expressing either an empty vector (VEC) or human MMP-14 cDNA (MT1) in monolayer growth conditions. MCF-7 cells were stably transfected with either an empty vector (pcDNA3.1/Zeo) or human MMP-14 cDNA (pcDNA3.1-MMP-14/Zeo). Cells were grown for 48 hours in monolayer culture. Cells were then lysed in TRIzol and total RNA was isolated. For each experimental condition, total RNAs isolated from 3 independant biological replicates were pooled.

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