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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of IL-18 generated murine NK cells

ABSTRACT: We analyzed gene expression profiles of IL-18 generated murine NK cells in comparison to unstimulated, freshly isolated splenic NK cells. We identified a set of 1414 Affymetrix probe sets showing significant misregulation (Welch's T-test, p<0.05; Benjamini-Hochberg FDR corrected). IL-18 generated as well as unstimulated NK cells were isolated in three independent preparations and used for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Rene Deenen

PROVIDER: E-GEOD-33627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice.

Ehlers Margret M Papewalis Claudia C Stenzel Wiebke W Jacobs Benedikt B Meyer Klaus L KL Deenen René R Willenberg Holger S HS Schinner Sven S Thiel Annette A Scherbaum Werner A WA Ullrich Evelyn E Zitvogel Laurence L Schott Matthias M

Endocrinology 20120625 9

Natural killer (NK) cells belong to the innate immune system. Besides their role in antitumor immunity, NK cells also regulate the activity of other cells of the immune system, including dendritic cells, macrophages, and T cells, and may, therefore, be involved in autoimmune processes. The aim of the present study was to clarify the role of NK cells within this context. Using two mouse models for type 1 diabetes mellitus, a new subset of NK cells with regulatory function was identified. These ce ...[more]

PMID: 22733969

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Transcription profiling of mouse resting and IL-15 activated natural killer cells

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2009-04-28 | E-GEOD-7764 | biostudies-arrayexpress

Differential requirement for Nfil3 during NK cell development

Project description:Natural killer (NK) cells can be grouped into distinct subsets that are localized to different organs and exhibit different capacity to secrete cytokines and mediate cytotoxicity. Despite these hallmarks that reflect tissue-specific specialization in NK cells, little is known about the factors that control the development of these distinct subsets. The basic leucine zipper transcription factor nuclear factor interleukin 3 (Nfil3; E4bp4) is essential for bone marrow-derived NK cell development but it is not clear whether Nfil3 is equally important for all NK cell subsets nor how it induces NK lineage commitment. Here we show that Nfil3 is required for the formation of Eomesodermin (Eomes)-expressing NK cells, including conventional medullary and thymic NK cells, whereas TRAIL+ Eomes- NK cells develop independent of Nfil3. Loss of Nfil3 during the development of bone marrow-derived NK cells resulted in reduced expression of Eomes and, conversely, restoration of Eomes expression in Nfil3-/- progenitors rescued NK cell development and maturation. Collectively, these findings demonstrate that Nfil3 drives the formation of mature NK cell by inducing Eomes expression and reveal the differential requirements of NK cell subsets for Nfil3. RNA-sequencing of natural killer (NK) cell subsets

2014-05-07 | E-GEOD-54291 | biostudies-arrayexpress

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2013-11-18 | E-GEOD-50122 | biostudies-arrayexpress

Cytokine-mediated natural killer cells effects impair hematopoietic stem cell function

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Next-generation sequencing identifies the natural killer cell microRNA transcriptome

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2014-02-01 | E-GEOD-43958 | biostudies-arrayexpress

Genome-wide maps of Runx3 bound regions in splenic NK cells activated in vivo with IL-15

Project description:ChIP-seq was conducted using freshly isolated splenic WT NK cells from IL-15/Ra treated mice with anti-Runx3 antibody (Ab) and non-immune serum (NIS) as control. Runx3 and NIS IP from splenic NK cells of IL-15/Ra treated WT mice, isolated by negative selection using NK cell isolation kit (R&D) followed by sorting of NKp46+ cells.

2014-01-22 | E-GEOD-50126 | biostudies-arrayexpress

Genome-wide maps of Runx3 bound regions in IL-2-activated splenic NK cells

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