Gene expression in articular cartilage - subchondral bone of FRZB knockout mice

ABSTRACT: Objective : To study molecular changes in the articular cartilage and subchondral bone of the tibial plateau from mice deficient in frizzled related protein (Frzb) compared to wild-type mice by transcriptome analysis. Methods : Gene-expression analysis of the articular cartilage and subchondral bone of 3 wild-type and 3 Frzb-/- mice was performed by microarray. Pathway analysis of differentially expressed genes between 3 wild-type and 2 Frzb-/- samples was explored with PANTHER, DAVID and GSEA bioinformatics tools. Activation of the WNT pathway was analyzed using western blot. The effects of Frzb gain and loss of function on chondrogenesis and cell proliferation was examined using ATDC5 micromasses and mouse ribcage chondrocytes. Results : Extracellular matrix-associated integrin and cadh

ORGANISM(S): Mus musculus

SUBMITTER: Joke Allemeersch 

PROVIDER: E-GEOD-33656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress