Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation

ABSTRACT: Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovered widespread promoter-centered intragenic, extragenic, and intergenic interactions. These interactions further aggregated into higher-order clusters, wherein proximal and distal genes were engaged through promoter-promoter interactions. Most genes with promoter-promoter interactions were active and transcribed cooperatively, and some interacting promoters could influence each other implying combinatorial complexity of transcriptional controls. Comparative analyses of different cell lines showed

ORGANISM(S): Homo sapiens

SUBMITTER: Guoliang Li 

PROVIDER: E-GEOD-33664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress