MicroRNA profiles of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice.

ABSTRACT: performed a genetic screen for microRNAs that are differentially expressed between LSK, LS−K+, erythroid and myeloid cells isolated from C57BL/6 and DBA/2 mice. This analysis identified 131 microRNAs that were differentially expressed between cell types and 15 that were differentially expressed between mouse strains. Of special interest was an evolutionary conserved miR-cluster located on chromosome 17 consisting of miR-99b, let-7e and miR-125a. All cluster members were most highly expressed in LSKs and down-regulated upon differentiation. In addition, these microRNAs were higher expressed in DBA/2 cells compared to C57BL/6 cells, and thus correlated with HSPC frequency. To functionally characterize these microRNAs, we overexpressed the entire miR-cluster 99b/let-7e/125a and miR-125a alone

ORGANISM(S): Mus musculus

SUBMITTER: Leonid Bystrykh 

PROVIDER: E-GEOD-33690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress