Differential regulation of SBF and MBF target genes by genotoxic stress occurs via Rad53-dependent inactivation of the MBF correpressor Nrm1

ABSTRACT: MBF and SBF transcription factors regulate a large family of coordinately expressed G1/S genes required for early cell cycle functions including DNA replication and repair. SBF is inactivated upon S phase entry by Clb/CDK whereas MBF targets are repressed by the corepressor, Nrm1. Using genome-wide expression analysis, we show that genotoxic stress during S phase specifically induces MBF-regulated genes via direct phosphorylation of Nrm1 by Rad53, the effector checkpoint kinase, and prevents its binding to MBF target promoters. Interestingly, some previously defined targets of SBF, including Swe1, are activated by replication stress. Swe1 undergoes a transcription factor exchange leading to its repression by Nrm1/MBF, which renders it sensitive to derepression by the S phase checkpoint. We

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Dwight Kuo 

PROVIDER: E-GEOD-33695 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress