TCERG1 regulates alternative splicing of Bcl-x gene by modulating the rate of RNAPII transcription

ABSTRACT: Complex functional coupling exists between transcriptional elongation and pre-mRNA alternative splicing. Pausing sites and changes in the rate of transcription by RNAPII may therefore have a fundamental impact in the regulation of alternative splicing. Here, we show that the elongation and splicing-related factor TCERG1 regulates alternative splicing of the apoptosis gene Bcl-x in a promoter-dependent manner. TCERG1 promotes the splicing of the short isoform of Bcl-x (Bcl-xs) through the SB1 regulatory element located in the first half of exon 2. Consistent with these results, we show evidence for in vitro and in vivo interaction of TCERG1 with the Bcl-x pre-mRNA. Transcription profile analysis reveals that the RNA sequences required for the effect of TCERG1 on Bcl-x alternative splicing c

ORGANISM(S): Homo sapiens

SUBMITTER: Marco Blanchette 

PROVIDER: E-GEOD-33716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress