Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of Drosophila mushroom bodies

ABSTRACT: We performed mRNA-seq of dissected Drosophila mushroom bodies, comparing to whole brain and testis mRNA seq of MB, brain and testis

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sidi Chen

PROVIDER: E-GEOD-33783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Frequent recent origination of brain genes shaped the evolution of foraging behavior in Drosophila.

Chen Sidi S Spletter Maria M Ni Xiaochun X White Kevin P KP Luo Liqun L Long Manyuan M

Cell reports 20120216 2

The evolution of the brain and behavior are coupled puzzles. The genetic bases for brain evolution are widely debated, yet whether newly evolved genes impact the evolution of the brain and behavior is vaguely understood. Here, we show that during recent evolution in Drosophila, new genes have frequently acquired neuronal expression, particularly in the mushroom bodies. Evolutionary signatures combined with expression profiling showed that natural selection influenced the evolution of young genes ...[more]

PMID: 22832161

Similar Datasets

Expression profiling of Drosophila mushroom bodies

Project description:We performed mRNA-seq of dissected Drosophila mushroom bodies, comparing to whole brain and testis

2012-02-01 | GSE33783 | GEO

Mushroom body cell type comparison

Project description:The Drosophila melanogaster mushroom bodies (MBs) are brain structures critical for olfactory memory. The approximately 2000 intrinsic MB neurons are divisible into alpha/beta neurons, alpha'/beta' neurons and gamma neurons by morphology and roles in memory processing. It has also been shown that the different subtypes of MB neurons have different function in the process of memory formation. This difference in function, suggested to us that different types of MB neurons might have different expression profiles. In this study, we used cell-type specific gene expression profiling to gain insight into cellular properties of MB neurons.

2013-01-10 | E-MEXP-3798 | biostudies-arrayexpress

Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

Project description:Temperature preference behavior in Drosophila depends on the level of PKA signaling in the mushroom bodies. To identify new components downstream to PKA, we carried out a genome-wide screen for genes regulated by PKA signaling in the mushroom bodies. Using the Gal4-UAS system, we increased or decreased PKA activity in the mushroom bodies by expressing dominant-negative (UAS-PKADN) or constitutively active PKA (UAS-PKACA), respectively. Expression of PKA transgenes was targeted to the mushroom bodies using the mushroom body-specific MB247-Gal4 driver. PKA expression was induced for 12-16 hours in three-day-old adults by inactivating the temperature-sensitive Gal80 at the restrictive temperature. We then analyzed gene-expression profiles to identify the genes showing altered expression levels in response to the high or low PKA activity.

2014-03-25 | E-GEOD-28181 | biostudies-arrayexpress

A Genome-Wide Survey of Drosophila Mushroom Body Transcripts with Chemical Ablation

Project description:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. To get insights of the repertoire of MB genes that control initiation and maintenance of neural differentiation as well as the repertoire of neural factors that may have functions in the synaptic plasticity of MB neurons during learning and memory, we compared the transcript profiles between wild type and MB-ablated brains using a Drosophila whole-genome microarray. Newly hatched larvae were briefly administered with a DNA-synthesis inhibitor, hydroxyurea, and raised to adults, from which total brain RNA was analyzed. Keywords: Chemical Ablation of Mushroom bodies from Drosophila brain

2006-07-01 | GSE3379 | GEO

Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

2014-03-25 | GSE28181 | GEO

Transcription profiling of Drosophila melanogaster ablated mushroom bodies vs unablated MB to investigate the repertoire of MB genes that control initiation and maintenance of neural differentiation during learning and memory

Project description:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. To get insights of the repertoire of MB genes that control initiation and maintenance of neural differentiation as well as the repertoire of neural factors that may have functions in the synaptic plasticity of MB neurons during learning and memory, we compared the transcript profiles between wild type and MB-ablated brains using a Drosophila whole-genome microarray. Newly hatched larvae were briefly administered with a DNA-synthesis inhibitor, hydroxyurea, and raised to adults, from which total brain RNA was analyzed. Experiment Overall Design: Two conditions analyzed: Control Brains and Musroom Body-ablated brains. Experiment Overall Design: Each condition was analyzed in triplicate.

2007-11-01 | E-GEOD-3379 | biostudies-arrayexpress

Nuclear transcriptomes of the seven neuronal cell types that constitute the Drosophila mushroom bodies

Project description:The insect mushroom body (MB) is a conserved central brain structure that receives multi-modal inputs and plays key roles in a diverse array of behaviors. In Drosophila melanogaster, the MB has served as the primary invertebrate model to investigate how neural circuits underlie plasticity related to memory formation and storage. A vast literature describes the development, morphology, wiring, and function of each of the MB cell types as well as their inputs and outputs. MBs consist of approximately 2000 intrinsic Kenyon Cells per hemisphere that are divided into three major neuron classes — γ, α′/β′ and α/β — based on their birth order, morphology and connectivity. These classes are further sub-divided into 7 cell subtypes — γd, γm, α′/β′ap, α′/β′m, α/βp, α/βs and α/βc. A wealth of functional studies demonstrate that these 7 MB cell subtypes connect to different input and output neurons and play distinct roles in memory processing. The functional differences between these cell subtypes are presumably reflected in unique transcriptional programs, although this has not been investigated. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each of the 7 MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes. These include the widely used α/β class marker Fas2 and the α′/β′ class marker trio. Immunostaining data corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptomic catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community.

2018-09-10 | GSE119629 | GEO

Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies

Project description:The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.

2022-10-19 | GSE216045 | GEO

Transcription profiling of Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator; This SuperSeries is composed of the following subset Series:; GSE10012: Timecourse: MB neurons at the onset and early steps of axon pruning; GSE10013: EcR-dependent gene expression in MB neurons at the onset of axon pruning Experiment Overall Design: Refer to individual Series

2008-06-18 | E-GEOD-10014 | biostudies-arrayexpress

Tissue-specific O-GlcNAcylation profiling reveals enrichment of ribosomal substrates in Drosophila mushroom body critical for associative learning

Project description:O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome with spatiotemporal precision in response to various stimuli. Its dysregulation is associated with many neurological disorders that impair cognitive function, and yet identification of phenotype-relevant protein substrates in specific brain regions remains unfeasible. By combining O-GlcNAc binding activity of Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific substrates enrichment. We mapped the O-GlcNAcylated proteome in different brain regions of Drosophila and revealed that components in translational machinery including many ribosomal subunits are heavily O-GlcNAcylated in mushroom body (MB), the major brain region for associative learning. Hypo-O-GlcNAcylation induced by ectopic expression of CpOGA in MB reduces ribosomal activity, leading to olfactory learning deficit. Our study provides a useful resource for dissecting tissue-specific functions of O-GlcNAcylation in Drosophila and suggests that new protein synthesis is important for cognitive function.

2024-04-10 | PXD040547 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data