Anaerobic oxidation of benzene by Geobacter metallireducens

ABSTRACT: Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance but research into the mechanisms has been stymied by a lack of a genetically tractable pure culture which unequivocally does not use molecular oxygen to activate benzene. Geobacter metallireducens grew in a medium in which benzene was the sole electron donor and Fe(III) was the sole electron acceptor with a stoichiometry of benzene loss and Fe(III) reduction consistent with benzene oxidation to carbon dioxide coupled with Fe(III) reduction. Phenol labeled with 18O was produced when the medium was labeled with H218O, as expected for a true anaerobic conversion of benzene to phenol. Gene expression patterns indicated that benzene was metabolized through a phenol intermediate rather th

ORGANISM(S): Geobacter metallireducens

SUBMITTER: Tian Zhang 

PROVIDER: E-GEOD-33794 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress