Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression levels of mature miRNAs from Huh7 cell treated with ROCK inhibitor

ABSTRACT: We used microarray to determine the miRNA whose expression was changed at 12 hours after Y27632 treatment Two-condition experiment; Huh7 control vs Huh7 treated with Y27632 for 12 hours

ORGANISM(S): Homo sapiens

SUBMITTER: Satoshi Kondo

PROVIDER: E-GEOD-33876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

The effects of apigenin on microRNA expression levels

Project description:The changes of mature microRNA expression levels after apigenin treatment on Huh7 cells were determined. Huh7 cells were treated with 10microM apigenin for 24hr and the changes of global mature microRNA expression levels were determined.

2013-08-20 | E-GEOD-46526 | biostudies-arrayexpress

Study of microRNA associated with cisplatin's anti-tumor effect in hepatocellular carcinoma

Project description:The miRNAs expression was markedly altered with the treatment of cisplatin in vitro and various miRNAs induced by cisplatin also may contribute to tumor growth in vitro. Using a custom microarray platform, we analyzed the expression levels of 1719 human miRNA probes in the cell lines in vitro that were treated with and without cisplatin.

2016-03-15 | E-GEOD-55806 | biostudies-arrayexpress

Study of microRNA associated with metforminM-bM-^@M-^Ys anti-tumor effect [Huh7 cells]

Project description:The miRNAs expression was markedly altered with the treatment of metformin in vitro and in vivo and various miRNAs induced by metformin also may contribute to tumor growth in vitro and in vivo. Using a custom microarray platform, we analyzed the expression levels of 985 human miRNA probes in the cell lines in vitro and tumorous tissues in vivo that were treated with and without metformin.

2015-03-01 | E-GEOD-44204 | biostudies-arrayexpress

Analysis of therapeutic miRNA against KRAS mutant colorectal cancer

Project description:We compared the profile of miRNAs expressed in HEK293 and MRC5 cells that overexpressed KRASG12V to those expressed in parental cells that harbored wild-type KRAS. The results indicated that a subset of miRNAs was significantly down-regulated in KRASG12V transfected two cells. To address the functional relevance of miRNAs in KRAS mutant cancers, we transfected exogenous KRASG12V into HEK293 and MRC5 cells with wild type KRAS genes, and we comprehensively profiled the dysregulated miRNAs.

2015-09-01 | E-GEOD-60370 | biostudies-arrayexpress

MicroRNA profile in hepatic epithelioid hemangioendothelioma

Project description:To elucidate the aetiology of HEH, especially with regard to microRNA (miRNA) profiles, samples obtained from three different parts of both normal and tumour tissues were analysed . The details of RNA extraction have been described in our previous reports. Interestingly, 16 miRNAs were significantly up-regulated and 51 miRNAs were down-regulated in the tumour tissues compared to the normal tissues (Studentâ??s t-test, p<0.01). In addition, unsupervised hierarchical clustering analysis using a Pearsonâ??s correlation showed that the tumour tissues clustered separately from the normal tissues. microRNA samples obtained from three different parts of both normal and tumour tissues were analysed

2016-03-15 | E-GEOD-66794 | biostudies-arrayexpress

Gene expression profiling of processing glycosidase inhibitors: defining a common gene set produced by processing glycosidase inhibition in human cervical carcinoma HeLa cells

Project description:The objective of the present study is to investigate the role of processing glycosidase inhibition induced by processing glycosidase inhibitors, namely castanospermin as the processing glucosidases inhibitor, deoxymannojirimycin as the processing mannosidases inhibitor, and deoxyfuconojirimycin as the fucosidase inhibitor. Comparative gene expression analysis was performed in human cervical carcinoma HeLa cells, with non-inhibitor-treated cells as the negative control. Gene expression was analyzed using 3D-GeneÂ® Human 25k oligonucleotide microarrays and computational gene expression analysis tools. Inhibition of processing glycosidases (glucosidases, mannosidases, and fucosidase) in human cervical carcinoma HeLa cells was performed by treating with a small molecule inhibitor and then culturing at 37Â°C. Total RNA samples were prepared from the cells. Gene expression was analyzed using 3D-GeneÂ® Human 25k oligonucleotide microarrays. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.

2014-09-01 | E-GEOD-40297 | biostudies-arrayexpress

SUV420H2 over-expression affects on gene expression in human breast cancer MDA-MB-231 cells

Project description:Analysis of breast cancer MDA-MB-231 cells stably over-expressing SUV420H2, a histone H4K20 methyltransferase. Several genes were significantly up- or down-regulated. Results provide insight into the molecular mechanism by which H4K20me3 contributes to gene expression. SUV420H2 stably over-expressing MDA-MB-231 cells were cloned. Then total RNA was extracted from the SUV420H2 over-expressing cells and the parental MDA-MB-231 cells.

2015-04-15 | E-GEOD-55947 | biostudies-arrayexpress

Effects of SUV39H1 depletion on gene expression in HeLa cells

Project description:HeLa cells were transiently transfected with siRNA against SUV39H1, a histone H3K9 methyltransferase. Several genes were significantly up- or down-regulated. HeLa cells were transiently transfected with siRNA against SUV39H1 or negative control siRNA. Then total RNA was extracted.

2015-08-01 | E-GEOD-59243 | biostudies-arrayexpress

Difference of microRNA expressions between a mouse model of NASH(MCD diet) and normal mouse.

Project description:The microRNAs expression was markedly altered with the MCD diet. Using a custom microarray platform, we analyzed the expression levels of 1135 mouse microRNA probes in liver tissue that were fed MCD diet.

2015-03-01 | E-GEOD-55593 | biostudies-arrayexpress

Comparison of miRNA expression profiling in CD14+ monocytes between anti-MDA5-positive three patients and three healthy controls

Project description:miRNA array was conducted to identify relevant pathological pathways in activated monocytes in anti-MDA5-positive patients in comparison of those in healthy controls.

2021-05-03 | E-MTAB-10471 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data