Comparison of TEX and M9-ENL1 cell lines to HL60 and THP1 cell lines

ABSTRACT: Gene regulatory networks that govern hematopoietic stem cells (HSC) and leukemia–initiating cells (L-IC) are deeply entangled. Thus, the discovery of compounds that target L-IC while sparing HSC is an attractive but difficult endeavor. Presently, most drug discovery approaches fail to counter-screen compounds against normal hematopoietic stem/progenitor cells (HSPC) to assess therapeutic index. Here, we present a combined in vitro and in vivo strategy to identify compounds specific to L-IC in acute myeloid leukemia (AML). A high-throughput screen of 4000 compounds on novel leukemia cell lines derived from human experimental leukemogenesis models yielded 80 hits, of which most were toxic to normal HSPC. Of the 10 compounds that passed this initial filter, we chose to characterize a single c

ORGANISM(S): Homo sapiens

SUBMITTER: Kolja Eppert 

PROVIDER: E-GEOD-33892 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress