Transcription profiling of rat ureteric buds treated with either heregulin, pleiotrophin or conditioned medium from metanephric mesenchymal cell culture

ABSTRACT: Genes ontologically classified as proliferative and developmental were down-regulated by HRG, whereas those involved in transport were up-regulated. For example, the mRNA for the GDNF receptors, GFRa1 and ret9 were down-regulated while the mRNA for collecting duct transporters, such as urea transporter2, aquaporin3, sodium-hydrogen exchanger2 were up-regulated in HRG-treated UBs compared with UBs grown in the presence of branch-promoting factors Experiment Overall Design: Total RNA was extracted from 3 isolated ureteric buds in culture per one treatment condition (1 sample). Three samples (biological replicates) were used for one condition.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hiroyuki Sakurai 

PROVIDER: E-GEOD-3394 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress