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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Role of PKD2 in TCR-induced transcriptional reprogramming of naïve T cells

ABSTRACT: Comparison of transcriptional profile of TCR stimulated P14-TCR wild-type and P14-PKD2 null murine lymph node cells Lymph node cells from 3 biological replicates (3 wild-type and 3 PKD2 null mice) were isolated and left unstimulated or stimulated for 4 hours with antigenic peptide (gp33-41) prior to RNA extraction and hybridization to Affymetrix microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Maria Navarro

PROVIDER: E-GEOD-33942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells.

Navarro Maria N MN Sinclair Linda V LV Feijoo-Carnero Carmen C Clarke Rosemary R Matthews Sharon A SA Cantrell Doreen A DA

The Biochemical journal 20120301 3

PKD (protein kinase D) 2 is a serine/threonine kinase activated by diacylglycerol in response to engagement of antigen receptors in lymphocytes. To explore PKD2 regulation and function in TCR (T-cell antigen receptor) signal transduction we expressed TCR complexes with fixed affinity for self antigens in the T-cells of PKD2-null mice or mice deficient in PKD2 catalytic activity. We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engag ...[more]

PMID: 22233340

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Role of PKD2 in TCR-induced transcriptional reprogramming of naïve T cells

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