Y. pestis KIM6+ TraSH Microarray Analysis After Intracellular Replication

ABSTRACT: The transposon site hybridization (TraSH) technique (Sassetti, CM et al. 2001. PNAS 98:12712-7) was utilized to identify genes important for the survival of Y. pestis within murine macrophages. A transposon library was created with ~31,500 Y. pestis KIM6+ insertion mutants. A portion of the Y. pestis transposon insertion mutant library was used to infect BMMs and the surviving bacteria (output pool) were recovered. TraSH was used to compare the output pool to a portion of the library that was not subjected to selection (input pool) in order to identify Y. pestis genes important for survival in macrophages. Each end of the transposon used for mutagenesis contains an outward-reading T7 RNA polymerase promoter. RNAs transcribed from the T7 promoters are complementary to the chromosomal D

ORGANISM(S): Yersinia pestis

SUBMITTER: Hana Fukuto 

PROVIDER: E-GEOD-33982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress