RNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts

ABSTRACT: Recent genome-wide chromatin immunoprecipitation coupled high throughput sequencing (ChIP-seq) analyses performed in various eukaryotic organisms, analysed RNA Polymerase II (Pol II) pausing around the transcription start sites of genes. In this study we have further investigated genome-wide binding of Pol II downstream of the 3' end of the annotated genes (EAGs) by ChIP-seq in human cells. At almost all expressed genes we observed Pol II occupancy downstream of the EAGs suggesting that Pol II pausing 3' from the transcription units is a rather common phenomenon. Downstream of EAGs Pol II transcripts can also be detected by global run-on and sequencing, suggesting the presence of functionally active Pol II. Based on Pol II occupancy downstream of EAGs we could distinguish distinct clusters

ORGANISM(S): Homo sapiens

SUBMITTER: Anamika Krishanpal 

PROVIDER: E-GEOD-34001 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress