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Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)

ABSTRACT: VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David L Parrott. The equivalent experiment is BB108 at PLEXdb.] treatment: Untreated Control(3-replications); treatment: Empty Virus (no inserted plant sequences)(3-replications); treatment: Rbaal VIGS(3-replications); treatment: Co-silencing (Rbaal + PDS)(3-replications)

ORGANISM(S): Hordeum vulgare

SUBMITTER: David Parrott

PROVIDER: E-GEOD-34102 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GmMPK4 is a negative regulator of SA-mediated defense response

Project description:GmMPK4 silencing was performed in Glycine max (Williams 82) using virus-induced gene silencing (VIGS) mediated by Bean pod mottle virus (BPMV). Microarray analysis was performed to compare the transcriptional profile of GmMPK4-silenced plants vs vector control plants. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jianzhong Liu. The equivalent experiment is GM25 at PLEXdb.] genotype: Williams 82(4-replications) genotype: GmMPK4-silenced Williams 82(4-replications)

2011-06-01 | E-GEOD-29653 | biostudies-arrayexpress

Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)

Project description:VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David L Parrott. The equivalent experiment is BB108 at PLEXdb.]

2011-12-03 | GSE34102 | GEO

Genotype-dependent gene expression in barley

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2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress

short term abiotic stress Cabernet Sauvignon

Project description:Cabernet Sauvignon plants grown in a hydroponic drip system were treat with 120 mM salt (10:1 NaCl:CaCl), Polyethylene Glycol, cold (5 C) or unstressed. Shoots with leaves were collected at 0, 1, 4, and 8 hours for all treatments, and at 24 hours for all except cold. ****[PLEXdb (http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Elizabeth Tattersall. The equivalent experiment is VV1 at PLEXdb.] time: 0 h - treatment: unstressed (3-replications); time: 1 h - treatment: unstressed (3-replications); time: 1 h - treatment: salt (3-replications); time: 1 h - treatment: PEG (3-replications); time: 1 h - treatment: cold (5 C) (3-replications); time: 4 h - treatment: unstressed (3-replications); time: 4 h - treatment: salt (3-replications); time: 4 h - treatment: PEG (3-replications); time: 4 h - treatment: cold (5 C) (3-replications); time: 8 h - treatment: unstressed (3-replications); time: 8 h - treatment: salt (3-replications); time: 8 h - treatment: PEG (3-replications); time: 8 h - treatment: cold (5 C) (3-replications); time: 24 h - treatment: unstressed (3-replications); time: 24 h - treatment: salt (3-replications); time: 24 h - treatment: PEG (3-replications)

2011-08-22 | E-GEOD-31594 | biostudies-arrayexpress

Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

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2011-11-22 | E-GEOD-33413 | biostudies-arrayexpress

Cross-species Detection in Barley1 GeneChip Array

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2011-11-22 | E-GEOD-33391 | biostudies-arrayexpress

Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line

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2011-11-28 | E-GEOD-29740 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33399 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33406 | biostudies-arrayexpress

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