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Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)

ABSTRACT: VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David L Parrott. The equivalent experiment is BB108 at PLEXdb.] treatment: Untreated Control(3-replications); treatment: Empty Virus (no inserted plant sequences)(3-replications); treatment: Rbaal VIGS(3-replications); treatment: Co-silencing (Rbaal + PDS)(3-replications)

ORGANISM(S): Hordeum vulgare

SUBMITTER: David Parrott

PROVIDER: E-GEOD-34102 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GmMPK4 is a negative regulator of SA-mediated defense response

Project description:GmMPK4 silencing was performed in Glycine max (Williams 82) using virus-induced gene silencing (VIGS) mediated by Bean pod mottle virus (BPMV). Microarray analysis was performed to compare the transcriptional profile of GmMPK4-silenced plants vs vector control plants. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jianzhong Liu. The equivalent experiment is GM25 at PLEXdb.] genotype: Williams 82(4-replications) genotype: GmMPK4-silenced Williams 82(4-replications)

2011-06-01 | E-GEOD-29653 | biostudies-arrayexpress

Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)

Project description:VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David L Parrott. The equivalent experiment is BB108 at PLEXdb.]

2011-12-03 | GSE34102 | GEO

Genotype-dependent gene expression in barley

Project description:Comparison of mRNA accumulation in the seedling leaves of 8 unstressed barley cultivars ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB20 at PLEXdb.] genotype: Morex(3-replications); genotype: Steptoe(3-replications); genotype: OWB REC(3-replications); genotype: OWB DOM(3-replications); genotype: Barke(3-replications); genotype: Haruna Nijo(3-replications); genotype: Golden Promise(3-replications); genotype: Optic(3-replications)

2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress

short term abiotic stress Cabernet Sauvignon

Project description:Cabernet Sauvignon plants grown in a hydroponic drip system were treat with 120 mM salt (10:1 NaCl:CaCl), Polyethylene Glycol, cold (5 C) or unstressed. Shoots with leaves were collected at 0, 1, 4, and 8 hours for all treatments, and at 24 hours for all except cold. ****[PLEXdb (http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Elizabeth Tattersall. The equivalent experiment is VV1 at PLEXdb.] time: 0 h - treatment: unstressed (3-replications); time: 1 h - treatment: unstressed (3-replications); time: 1 h - treatment: salt (3-replications); time: 1 h - treatment: PEG (3-replications); time: 1 h - treatment: cold (5 C) (3-replications); time: 4 h - treatment: unstressed (3-replications); time: 4 h - treatment: salt (3-replications); time: 4 h - treatment: PEG (3-replications); time: 4 h - treatment: cold (5 C) (3-replications); time: 8 h - treatment: unstressed (3-replications); time: 8 h - treatment: salt (3-replications); time: 8 h - treatment: PEG (3-replications); time: 8 h - treatment: cold (5 C) (3-replications); time: 24 h - treatment: unstressed (3-replications); time: 24 h - treatment: salt (3-replications); time: 24 h - treatment: PEG (3-replications)

2011-08-22 | E-GEOD-31594 | biostudies-arrayexpress

Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

Project description:Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Aravind Jukanti. The equivalent experiment is BB53 at PLEXdb.] developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications)

2011-11-22 | E-GEOD-33413 | biostudies-arrayexpress

Cross-species Detection in Barley1 GeneChip Array

Project description:This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB1 at PLEXdb.] species: Barley(2-replications); species: Wheat(2-replications); species: Oat(2-replications); species: Sorghum(2-replications); species: Corn(2-replications); species: Rice(2-replications)

2011-11-22 | E-GEOD-33391 | biostudies-arrayexpress

Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line

Project description:Experimental design: 1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene) 3 treatments: Virulent soybean rust (Phakopsora pachyrhizi Tw80-2) challenge, avirulent soybean rust challenge (Hw94-1) & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 54 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94 treatment: 500,000 spores per ml in 0.01% Tween 20 Taiwan 80-2 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel (for Steve Whitham). The equivalent experiment is GM36 at PLEXdb.] pathogen isolates: Mock - time: 12 hai(3-replications); pathogen isolates: Mock - time: 24 hai(3-replications); pathogen isolates: Mock - time: 72 hai(3-replications); pathogen isolates: Mock - time: 144 hai(3-replications); pathogen isolates: Mock - time: 216 hai(3-replications); pathogen isolates: Mock - time: 288 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 12 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 24 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 72 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 144 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 216 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 288 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 12 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 24 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 72 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 144 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 216 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 288 hai(3-replications)

2011-11-28 | E-GEOD-29740 | biostudies-arrayexpress

Microarray of barley genes in wheat-barley chromosome addition lines

Project description:Transcript levels of barley genes were examined in the wheat-barley chromosome addition lines having one of six barley chromomes, 2H, 3H, 4H, 5H, 6H and 7H. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is BB8 at PLEXdb.] genotype: chr2H(3-replications); genotype: chr2HL(3-replications); genotype: chr3H(3-replications); genotype: chr4H(3-replications); genotype: chr5H(3-replications); genotype: chr6H(3-replications); genotype: chr6HL(3-replications); genotype: chr6HS(3-replications); genotype: chr7H(3-replications); genotype: Betzes(3-replications); genotype: Chinese Spring(3-replications)

2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

Necrotic mutants in barley cv. Steptoe

Project description:FN044, FN211, FN242 and FN303 are the fast neutron generated mutants in cv. Steptoe background. These 4 mutants have lesion mimic phenotype and increase disease resistance to stem rust. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, ling zhang. The equivalent experiment is BB54 at PLEXdb.] line: nad1-1(3-replications); line: nad1-2(3-replications); line: nad1-3(3-replications); line: nad1-4 (FN242)(3-replications); line: Steptoe(3-replications)

2011-11-22 | E-GEOD-33399 | biostudies-arrayexpress

mlo5-mediated resistance responses in barley

Project description:Gene expression of a near-isogenic pair of barley lines differing in absence or presence of the mlo5 resistance gene in response to attack by barley powdery mildew (Bgh). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Achim Fischer. The equivalent experiment is BB7 at PLEXdb.] disease type: Control - genotype: Mlo(1-replications); disease type: Control - genotype: mlo5(1-replications); disease type: Treated (Pathogen Infection) - genotype: Mlo(1-replications); disease type: Treated (Pathogen Infection) - genotype: mlo5(1-replications)

2011-11-22 | E-GEOD-33406 | biostudies-arrayexpress

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