Multicolor miRNA fluorescence in situ hybridization for tumor differential diagnosis
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Neil Renwick
PROVIDER: E-GEOD-34137 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Multicolor microRNA FISH effectively differentiates tumor types.
Renwick Neil N Cekan Pavol P Masry Paul A PA McGeary Sean E SE Miller Jason B JB Hafner Markus M Li Zhen Z Mihailovic Aleksandra A Morozov Pavel P Brown Miguel M Gogakos Tasos T Mobin Mehrpouya B MB Snorrason Einar L EL Feilotter Harriet E HE Zhang Xiao X Perlis Clifford S CS Wu Hong H Suárez-Fariñas Mayte M Feng Huichen H Shuda Masahiro M Moore Patrick S PS Tron Victor A VA Chang Yuan Y Tuschl Thomas T
The Journal of clinical investigation 20130601 6
MicroRNAs (miRNAs) are excellent tumor biomarkers because of their cell-type specificity and abundance. However, many miRNA detection methods, such as real-time PCR, obliterate valuable visuospatial information in tissue samples. To enable miRNA visualization in formalin-fixed paraffin-embedded (FFPE) tissues, we developed multicolor miRNA FISH. As a proof of concept, we used this method to differentiate two skin tumors, basal cell carcinoma (BCC) and Merkel cell carcinoma (MCC), with overlappin ...[more]
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