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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

ABSTRACT: miRNAs play an important role in regulating CD8+ T cell response. We used the microarray approach to profile the miRNA signatures of naïve, day 5 effector, day 8 effector, and memory CD8+ T cells. We identified a miRNA signature associated with rapidly proliferating effector CD8+ T cells. CD8+ T cells from P14 TCR transgenic mice were transferred to C57BL6 recipients which were subsequently infected with LCMV Armstrong. Donor P14 CD8+ T cells were sorted on day 5, day 8, or >day 60 post-infection. Naïve P14 CD8+ T cells were sorted directly from naive P14 splenocytes. The total RNA including miRNAs was extracted from sorted samples, labeled, and hybridized to Agilent Mouse miRNA microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Tuoqi Wu

PROVIDER: E-GEOD-34216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Temporal expression of microRNA cluster miR-17-92 regulates effector and memory CD8+ T-cell differentiation.

Wu Tuoqi T Wieland Andreas A Araki Koichi K Davis Carl W CW Ye Lilin L Hale J Scott JS Ahmed Rafi R

Proceedings of the National Academy of Sciences of the United States of America 20120604 25

MicroRNAs are important regulators of various developmental and physiological processes. However, their roles in the CD8(+) T-cell response are not well understood. Using an acute viral infection model, we show that microRNAs of the miR-17-92 cluster are strongly induced after T-cell activation, down-regulated after clonal expansion, and further silenced during memory development. miR-17-92 promotes cell-cycle progression of effector CD8(+) T cells, and its expression is critical to the rapid ex ...[more]

PMID: 22665768

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Temporal expression of miR-17-92a regulates effector and memory CD8+ T cell differentiation

Project description:This SuperSeries is composed of the following subset Series: GSE34216: miRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection GSE34217: Expression profile of miR-17-92a-MSCV-IRES-Thy1.1 transduced P14 CD8+ T cells Refer to individual Series

2012-06-04 | E-GEOD-34218 | biostudies-arrayexpress

Expression profile of miR-17-92a-MSCV-IRES-Thy1.1 transduced P14 CD8+ T cells

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2012-06-04 | E-GEOD-34217 | biostudies-arrayexpress

Transcription profiling of mouse IL-7Rhi/lo effector CD8 T cells on day 6/7 after LCMV armstrong infection

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2008-06-16 | E-GEOD-8678 | biostudies-arrayexpress

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2011-08-22 | E-GEOD-30962 | biostudies-arrayexpress

Gene expression analysis of Wnt+ and Wnt- effector CD8 T cells

Project description:Wnt signal transduction during an immune response is involved in the establishment of functional CD8 T cell memory P14 ConductinLacZ CD8 T cells (CD45.2) were transferred in CD45.1+.2+ recipient mice. The day after, recipients were infected with 200pfu LCMV WE. At day 8 after infection, cells from spleen and lymph nodes were harvested, magnetically enriched for CD8 T cells, loaded with the beta-galactosidase substrate (FDG) and sorted as 7AAD-negative CD45.- negative, beta-galactosidase positive versus negative cells. P14 CD8 T cells were sorted at day 8 after LCMV infection according to LacZ activity for RNA extraction and hybridization on Affymetrix microarrays.

2013-12-16 | E-GEOD-53358 | biostudies-arrayexpress

Global DNA Methylation Remodeling Accompanies CD8 T Cell Effector Function

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2013-08-18 | E-GEOD-44638 | biostudies-arrayexpress

Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells

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2012-03-01 | E-GEOD-36168 | biostudies-arrayexpress

The effect of type-I interferon signaling directly on CD8+ T cells following LCMV infection in the presence or absence of NK cells.

Project description:To understand the effect of type-I IFN signaling directly on CD8+ T cells following an LCMV infection we compared the overall gene expression of WT and Ifnar1-/- T cells following an LCMV infection. WT and Ifnar1-/- P14 cells were sorted from infected mice (day 3) that were depleted of NK cells or left untreated. Each sample and condition (WT or IFNAR) was performed in triplicates

2014-05-07 | E-GEOD-57355 | biostudies-arrayexpress

miRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

Project description:miRNAs play an important role in regulating CD8+ T cell response. We used the microarray approach to profile the miRNA signatures of naïve, day 5 effector, day 8 effector, and memory CD8+ T cells. We identified a miRNA signature associated with rapidly proliferating effector CD8+ T cells.

2012-06-05 | GSE34216 | GEO

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