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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Dorsal dependent CBP binding on a genome wide scale

ABSTRACT: We find a high concordance between the binding of the Drosophila transcription factor Dorsal and the co-activator CBP during early embryogenesis. This relationship was furter examined by comparing CBP distribution in Drosophila embryos derived from wt and mutant flies lacking intranuclear Dorsal (gd7). Our data suggests a specific involvemet of CBP in initiating early dorsoventral patterning, but not in anterioposterior. CBP ChIP seq of 2-4 hours old Drosophila embryos derived from w1118 (wild-type) or gd7 homozygous mutant mothers

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mattias Mannervik

PROVIDER: E-GEOD-34221 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Preferential genome targeting of the CBP co-activator by Rel and Smad proteins in early Drosophila melanogaster embryos.

Holmqvist Per-Henrik PH Boija Ann A Philip Philge P Crona Filip F Stenberg Per P Mannervik Mattias M

PLoS genetics 20120621 6

CBP and the related p300 protein are widely used transcriptional co-activators in metazoans that interact with multiple transcription factors. Whether CBP/p300 occupies the genome equally with all factors or preferentially binds together with some factors is not known. We therefore compared Drosophila melanogaster CBP (nejire) ChIP-seq peaks with regions bound by 40 different transcription factors in early embryos, and we found high co-occupancy with the Rel-family protein Dorsal. Dorsal is requ ...[more]

PMID: 22737084

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