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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis leaf gene expression after growth under low light and low CO2

ABSTRACT: Internal sugar and light specific dependent regulation of leaf gene expression was addressed by changing [CO2] to lower than compensation point [CO2] in combination with light or prolonged darkness. Plants were grown on soil in a 12/12 h light/dark rhythm at 20Â°C day and night and under normal [CO2]. 5 weeks after germination, the above-ground rosettes of the non-flowering plants were harvested, 12 plants per sample. Plants were harvested 4hrs after the end of night (i) under low (< 50 ppm) [CO2] and 150 ÂµE fluorescent light , (ii) under normal [CO2] and light, and, (iii) under low [CO2] and prolonged darkness. The low [CO2] treatment started 30 min before the end of night and stopped with harvesting. Experiment Overall Design: 2 biological replicates of plants under 3 treatments (4hrs low CO2+light, 4hrs low CO2+prolonged darkness, and 4hrs normal CO2+light).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Oliver Bläsing

PROVIDER: E-GEOD-3423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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