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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea

ABSTRACT: The transcriptome of the planarian Schmidtea mediterranea is not well characterized. We have used RNA-Seq to characterize the transcriptome in both sexual and asexual strains of S. mediterranea from both untreated and irradiated animals. Moreover, we have performed RNA-Seq on RNA purified from FACS sorted neoblasts and differentiated cells. Together these studies expand our understanding of the planarian transcriptome and have identified strain-specific, neoblast-specific, and conserved transcripts. RNA-Seq was performed on RNA isolated from untreated and irradiated S. mediterranea animals from both the sexual and asexual strains, on FACS purified X1 neoblasts, X2 neoblasts, and Xins differentiated cells. One of the raw data files for GSM847465 is missing. The fasta file is provided at http://genome.vcell.uchc.edu/GenomeData02/Graveley_Lab_Public_Data/Planarian/S.mediterranea_SexNIRmRNA3.fa.gz

ORGANISM(S): Schmidtea mediterranea

SUBMITTER: Brenton Graveley

PROVIDER: E-GEOD-34326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea.

Resch Alissa M AM Palakodeti Dasaradhi D Lu Yi-Chien YC Horowitz Michael M Graveley Brenton R BR

PloS one 20120404 4

The planarian Schmidtea mediterranea is a powerful model organism for studying stem cell biology due to its extraordinary regenerative ability mediated by neoblasts, a population of adult somatic stem cells. Elucidation of the S. mediterranea transcriptome and the dynamics of transcript expression will increase our understanding of the gene regulatory programs that regulate stem cell function and differentiation. Here, we have used RNA-Seq to characterize the S. mediterranea transcriptome in sex ...[more]

PMID: 22496805

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