Modulation of NF-kB-Dependent Gene Transcription Using Programmable DNA Minor Groove Binders

ABSTRACT: Nuclear factor kappaB (NF-kB) is a transcription factor that regulates various aspects of immune response, cell death and differentiation as well as cancer. In this study we introduce the Py-Im polyamide 1 that binds preferentially to the sequences 5'-WGGWWW-3' and 5' GGGWWW-3'. The compound is capable of binding to kB sites and reducing the expression of various NF-kB driven genes including IL6 and IL8 by qRT-PCR. Chromatin immunoprecipitation experiments demonstrate a reduction of p65 occupancy within the proximal promoters of those genes. Genome-wide expression analysis by RNA-seq compares the DNA-binding polyamide with the well-characterized NF-kB inhibitor PS1145, identifying overlaps and differences in affected gene groups and showing that both affect comparable numbers of TNFa induc

ORGANISM(S): Homo sapiens

SUBMITTER: Jevgenij Raskatov 

PROVIDER: E-GEOD-34329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress