Expression analysis of Drosophila melanogaster's primordial germ cells and somatic cells in wild type and smaug mutants

ABSTRACT: Background: During the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have been restricted to whole embryos. Here we report the first such analysis for primordial germ cells (PGCs), the progenitors of the germ-line stem cells. Results: We purified PGCs from Drosophila embryos, defined their proteome and transcriptome, and assessed the content, scale and dynamics of their MZT. Transcripts encoding proteins that implement particular types of biological functions group into nine distinct expression profiles, reflecting coordinate control at the transcriptional and posttrans

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Howard Lipshitz 

PROVIDER: E-GEOD-34397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress