Gene expression analysis of wild type and KAP1 KO mouse T cell progenitors

ABSTRACT: The modulation of chromatin status at specific genomic loci controls lymphoid differentiation. Here, we investigated the role played in this process by KAP1, the universal cofactor of KRAB-containing Zinc Finger Proteins (KRAB-ZFPs), a tetrapod-restricted family of transcriptional repressors. T cell-specific Kap1 knockout mice displayed a significant expansion of immature thymocytes and imbalances in the ratios of mature T cells in the thymus and the spleen, with impaired responses to TCR stimulation. Transcriptome and chromatin studies revealed that KAP1 directly controls the expression of a number of genes involved in TCR and cytokine signalling, among which Traf1 and FoxO1, and is strongly associated with cis-acting regulatory elements marked by the H3K9me3 repressive mark on the genome

ORGANISM(S): Mus musculus

SUBMITTER: Francesca Santoni de Sio 

PROVIDER: E-GEOD-34447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress