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Gene expression profiles of human CD1c+ CD19- blood dendritic cells [human data]

ABSTRACT: In this study gene expression of CD1c+ CD19- blood dendritic cells from healthy subjects were investigated. Keywords: expression profiling by array Blood DCs (CD1c+ CD19-) were isolated from venous heparinized blood of three apparently healthy human volunteers using the CD1c+ (BDCA-1) dendritic cell isolation kit from Miltenyi according to the manufacturer’s instructions. Purity over 90% (95.8±3%) was determined by FACS analysis. RNA was isolated and 1 µg was labeled using the Affymetrix One-Cycle Target Labeling Kit and the biotinylated cRNA used to perform Affymetrix U133 Plus 2.0 expression arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Olivia Prazeres da Costa

PROVIDER: E-GEOD-34515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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