Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiles in Arabidopsis plants during the treatment of AgNO3.

ABSTRACT: Camalexin, an indolic secondary metabolite, is the magor phytoalexin produced by Arabidopsis thaliana. Camalexin biosynthesis is induced by abiotic stresses such as heavy metal treatment (e.g AgNO3). With the aim of identifying positive regulators of camalexin biosynthesis in Arabidopsis, we listed putative transcription factor genes strongly induced during the time course of AgNO3 treatment from a transcriptome analysis. AgNO3 induced gene expression in Arabidopsis was measured at 48 and 72 hours after the 0.1 mM AgNO3 treatment. Arabidopsis seedlings were germinated under sterile conditions on GM-agar plates. Seedlings (one-week after germination) were then transferred to GM liquid culture and shaken for two weeks. Plants were then treated with 0.1 mM AgNO3 and sampled at 0 h, 48 h and 72 h after the treatment. Three independent experiments were performed at each time (0, 48, or 72 h) using different plants for each experiment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hirohisa Saga

PROVIDER: E-GEOD-34606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Nanoparticles are compounds of emerging concern with largely unknown risks for human and ecological health. It is crucial to evaluate their potential biological impact to prevent unintended adverse effects on human health and the environment. We analyzed the transcriptional effects of polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) and silver nitrate (AgNO3) on the fathead minnow (Pimephales promelas) to understand their potential toxicity and adverse outcomes. We also tested the feasibility of the fathead minnow as an alternative species to elucidate potential adverse effects on humans. Fathead minnow females were exposed to either 4 Âµg/L of AgNO3 or 70 Âµg/L of PVP-AgNPs for 96h. Microarray analyses were performed on liver and brain. Functional analysis identified potential toxicity pathways and molecular initiating events (MIEs) that were confirmed with functional assays. Data suggested that AgNO3 and PVP-AgNPs had both common and distinct transcriptional effects. The nanoparticles were linked to neurotoxicity and oxidative stress, and identified as a dopamine receptor antagonist. Silver nitrate was also identified as a potential neurotoxicant and was confirmed as adrenergic and cannabinoid receptors antagonist. While silver nitrate and PVP-AgNPs were both potential neurotoxicants, they appeared to act through different MIEs. Fathead minnow is a promising alternative species to elucidate potential adverse effects of relevance to human health. We analyzed the transcriptional effects of polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) and silver nitrate (AgNO3) on the fathead minnow (Pimephales promelas) to understand their potential toxicity and adverse outcomes. FHM were obtained from Aquatic Biosystems (Fort Collins, CO), held in aerated dechlorinated tap water and fed three times daily with ZeiglerÂ® AquaTox Feed Gardners, PA, USA). Fathead minnow females were exposed to either 4 Âµg/L of AgNO3 or 70 Âµg/L of PVP-AgNPs (Luna Innovations, Blackburn, VA) for 96h at 24Â°C Â± 1 with a 90% water change at 48 hours. Microarray analyses were performed on liver and brain.

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Gene expression profiles in Arabidopsis plants during the treatment of AgNO3.

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