Airborne particulate matter affects gene expression in BEAS-2B bronchial epithelial cells.

ABSTRACT: Background: In classrooms high concentrations of particulate matter PM10 were measured. It is unknown whether the hazard of indoor particles is similar to that of the better studied outdoor particles. This study therefore analyzed adverse biological effects of classroom in comparison to outdoor PM10. Methods: Samples were taken from six schools during teaching hours. Genome-wide gene expression in human bronchial BEAS-2B epithelial cells was analyzed, and regulated genes were verified by quantitative PCR. Polycyclic aromatic hydrocarbons (PAH), endotoxin, and cat allergen Fel d 1 were analyzed with standard methods. Enhancement of allergic reactivity by PM10 was confirmed with CD63 upregulation in human primary basophils. Acceleration of human blood coagulation was determined with supern

ORGANISM(S): Homo sapiens

SUBMITTER: Roland Lang 

PROVIDER: E-GEOD-34607 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress