Differential gene expression profile of Streptococcus pneumoniae early biofilm cells and planktonic cells

ABSTRACT: Otitis media, pneumonia, sinusitis and as well as severe diseases such as meningitis and bacteraemia are related to biofilm-like diseases, in which Streptococcus pneumoniae demonstrated differential and tissue specific gene expressions. In this study, we reported the differential gene expression profile of early in vitro biofilm and planktonic cell in c-DNA microarray analysis. The microarray analysis was performed on total RNA extracted from biofilms grown in 24-well microtiter plate and mid-log grown planktonic cells. To validate the results of microarray, real-time RT-PCR was performed on 13 differentially expressed genes and one constitutively expressed gene from six different functional groups. cDNA-microarray analyses indicated 89 genes that were significantly differentially expresse

ORGANISM(S): Streptococcus pneumoniae R6

SUBMITTER: Jae-Jun Song 

PROVIDER: E-GEOD-34751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress