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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of genes characteristic of primary inguinal or epididymal preadipocyte fibroblasts

ABSTRACT: Committed preadipocyte fibroblasts were genetically labelled in transgenic mice by expressing GFP under the control of the locus for Zfp423, a gene controlling preadipocyte determination. These mice are herein referred to as Zfp423-GFP mice. The overall goal was to identify genes differentially expressed between adipogenic GFP+ firboblasts and non-adipogenic GFP- fibroblasts from either inguinal or epididymal fat stromal vascular cultures obtained from Zfp423-GFP mice. Adipose stromal vascular cultures were obtained from inguinal or epididymal fat of 6-8 week-old male Zfp423-GFP mice. GFP+ and GFP- fibroblasts were sorted from fat stromal vascular cultures and then returned to culture. Subconfluent cultures of GFP+ and GFP- cells were compared in triplicate for Affymetrix analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Rana Gupta

PROVIDER: E-GEOD-34775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes characteristic of primary inguinal or epididymal preadipocyte fibroblasts

Project description:Committed preadipocyte fibroblasts were genetically labelled in transgenic mice by expressing GFP under the control of the locus for Zfp423, a gene controlling preadipocyte determination. These mice are herein referred to as Zfp423-GFP mice. The overall goal was to identify genes differentially expressed between adipogenic GFP+ firboblasts and non-adipogenic GFP- fibroblasts from either inguinal or epididymal fat stromal vascular cultures obtained from Zfp423-GFP mice.

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Gene expression of adipocyte and stromal-vascular fractions

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