"Calling Cards" for DNA-binding proteins in mammalian cells

ABSTRACT: The ability to chronicle transcription factor binding events throughout the development of an organism would facilitate mapping of transcriptional networks that control cell fate decisions. We describe a method for permanently recording protein-DNA interactions in mammalian cells. We endow transcription factors with the ability to deposit a transposon into the genome near to where they bind. The transposon becomes a M-bM-^@M-^\Calling CardM-bM-^@M-^] the transcription factor leaves behind to record its visit to the genome. The locations of the Calling Cards can be determined by massively-parallel DNA sequencing. We show that the transcription factor SP1 fused to the piggyBac transposase directs insertion of the piggyBac transposon near SP1 binding sites. The locations of transposon insert

ORGANISM(S): Homo sapiens

SUBMITTER: David Mayhew 

PROVIDER: E-GEOD-34791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress