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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Stem cell lines of the early mouse embryo

ABSTRACT: Expression profiling of stem cell lines derived from the early embryo representing the trophoblast, primitive endoderm, early epiblast (inner cell mass E3.5) and late post-implantation epiblast (E5.5). Cells were grown without feeders and harvested. Comparisons were made to provide evidence of unique gene expression between the cell lines. Specifically, pre-implantation (ICM, epiblast and primitive endoderm, and trophoblast), as well as pre-implantation and post-implantation epiblasts.

ORGANISM(S): Mus musculus

SUBMITTER: Brian Cox

PROVIDER: E-GEOD-34799 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.

Rugg-Gunn Peter J PJ Cox Brian J BJ Lanner Fredrik F Sharma Parveen P Ignatchenko Vladimir V McDonald Angela C H AC Garner Jodi J Gramolini Anthony O AO Rossant Janet J Kislinger Thomas T

Developmental cell 20120315 4

The advent of reprogramming and its impact on stem cell biology has renewed interest in lineage restriction in mammalian embryos, the source of embryonic (ES), epiblast (EpiSC), trophoblast (TS), and extraembryonic endoderm (XEN) stem cell lineages. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-sc ...[more]

PMID: 22424930

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