CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

ABSTRACT: The functional shift of quiescent endothelial cells into tip cells that migrate and stalk cells that proliferate is a key event during sprouting angiogenesis. We previously showed that the sialomucin CD34 is expressed in a small subset of cultured endothelial cells and that these cells extend filopodia: a hallmark of tip cells in vivo. In the present study, we characterized endothelial cells expressing CD34 in endothelial monolayers in vitro. We found that CD34-positive human umbilical vein endothelial cells show low proliferation activity and increased mRNA expression of all known tip cell markers, as compared to CD34- negative cells. Genome-wide mRNA profiling analysis of CD34-positive endothelial cells demonstrated enrichment for biological functions related to angiogenesis and migratio

ORGANISM(S): Homo sapiens

SUBMITTER: Ingeborg Klaassen 

PROVIDER: E-GEOD-34850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress