Induced pluripotent stem cell-derived models of sporadic and familial Alzheimer’s disease

ABSTRACT: Our understanding of Alzheimer’s disease (AD) pathogenesis is currently limited by difficulties in obtaining live neurons from patients and the inability to model the sporadic form of AD. It may be possible to overcome these challenges by reprogramming primary cells from patients into induced pluripotent stem cells (iPSCs). We reprogrammed primary fibroblasts from two patients with familial AD (both caused by a duplication of APP1, APPDp), two with sporadic AD (sAD1, 2) and two non-demented control individuals (NDCs) into iPSC lines. Neurons from differentiated cultures were FACS-purified and characterized. Purified cultures contained >90% neurons, clustered with fetal brain mRNA samples by microarray criteria, and could form functional synaptic contacts. Virtually all cells exhibited norm

ORGANISM(S): Homo sapiens

SUBMITTER: Kristopher Nazor 

PROVIDER: E-GEOD-34879 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress