Transcription profiling of human brain samples from HIV infected individuals with HIV encephalitis vs controls

ABSTRACT: The neurodegenerative process in HIV encephalitis (HIVE) is associated with extensive damage to the dendritic and synaptic structure that often leads to cognitive impairment. Several mechanisms might be at play, including release of neurotoxins, oxidative stress and decreased activity of neurotrophic factors. Furthermore, HIV-mediated dysregulation of genes involved in neuronal maintenance might play an important role. For this purpose, cRNA was prepared from the brains of 17 AIDS patients for analysis with the Affymetrix Human U95Av2 GeneChip and analyzed with the GeneSpring Expression Analysis Software. Out of 12,625 genes analyzed, 74 were downregulated and 59 were upregulated compared to controls. Initial alternative analysis of RNA was performed by ribonuclease protection assay (RPA).

ORGANISM(S): Homo sapiens

DISEASE(S): HIV encephalitis

SUBMITTER: Jennifer Hammond 

PROVIDER: E-GEOD-3489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress