Gene expression from Inducible TOC1 expression in Arabidopsis seedlings

ABSTRACT: The first described feedback loop of the Arabidopsis circadian clock is based on reciprocal regulation between TOC1 and CCA1/LHY. CCA1 and LHY are MYB transcription factors that bind directly to the TOC1 promoter to negatively regulate its expression. Conversely, the activity of TOC1 has remained less well characterized. Genetic data supports that TOC1 is necessary for the reactivation of CCA1/LHY, but there is little description of its biochemical function. Here we show that TOC1 occupies specific genomic regions in the CCA1 and LHY promoters. Purified TOC1 binds directly to DNA through its CCT domain, which is similar to known DNA binding domains. Chemical induction and transient overexpression of TOC1 in Arabidopsis seedlings cause repression of CCA1/LHY expression demonstrating that TO

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Colleen Doherty 

PROVIDER: E-GEOD-34897 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress