Defective uncoupled differentiations after nuclear reprogramming

ABSTRACT: Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. At Day 18, 30 SCNT conceptuses were compared to 20 controls (AI and IVP: 10 conceptuses each); one-half of the SCNT conceptuses appeared normal while the other half showed signs of atypical elongation and gastrulation. SCNT was also associated with a high incidence of discordan

ORGANISM(S): Bos taurus

SUBMITTER: Isabelle Hue 

PROVIDER: E-GEOD-34944 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress