Chronic colitis in Bacteroides Thetaiotaomicron-Monoassociated HLA-B27 Transgenic rats alters expression of receptor and metabolic genes in luminal bacteria.

ABSTRACT: Background: Inflammatory bowel diseases (IBD) may be caused in part by aberrant immune responses to commensal intestinal microbes including Bacteroides thetaiotaomicron (B.theta). Healthy, germ-free HLA-B27 transgenic (Tg) rats develop chronic colitis when colonized with complex gut commensal bacteria whereas non-transgenic (nTg) rats remain disease-free. However, the role of B.theta, a well-characterized anaerobic commensal bacterium, in causing disease in Tg rats is unknown nor is much known about how microbes respond to host inflammation. Methods: Tg and nTg rats were monoassociated with a human isolate of B.theta. Colonic inflammation was quantified by blinded histological scoring and real-time RT-PCR assays of pro-inflammatory cytokines. Cecal bacterial concentrations were measure

ORGANISM(S): Bacteroides thetaiotaomicron

SUBMITTER: Yong Huang 

PROVIDER: E-GEOD-34966 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress