Post-transcriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in S. pombe

ABSTRACT: Members of the tristetraprolin (TTP) family of CCCH tandem zinc finger proteins can bind directly to AU-rich elements in mRNAs and promote transcript deadenylation and decay. The yeast Schizosaccharomyces pombe expresses a single TTP family member, Zfs1p, that has been linked to the mating response pathway and septum formation. We showed previously that Zfs1p can bind to and promote the destabilization of AU-rich element-containing transcripts. In this study, we identified additional target transcripts by comparing transcript levels in wild type and zfs1 mutant yeast, using deep sequencing and microarray approaches. We also used direct RNA sequencing to determine the locations of the polyA tails in both wild type and mutant strains, and to confirm the presence of potential Zfs1p target

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: NIEHS Microarray Core 

PROVIDER: E-GEOD-35009 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress