Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells

ABSTRACT: Two populations of Nkx2-1+ progenitors in the developing foregut endoderm give rise to the entire post-natal lung and thyroid epithelium, but little is known about these cells, as they are difficult to isolate in a pure form. We demonstrate here the purification and directed differentiation of primordial lung and thyroid progenitors derived from mouse embryonic stem cells (ESCs). Inhibition of TGFM-NM-2 and BMP signaling, followed by combinatorial stimulation of BMP and FGF signaling can specify these cells efficiently from definitive endodermal precursors. When derived using Nkx2-1GFP knock-in reporter ESCs, these progenitors can be purified for expansion in culture and have a transcriptome that overlaps with developing lung epithelium. Upon induction, they can express a broad repertoire

ORGANISM(S): Mus musculus

SUBMITTER: steve shen 

PROVIDER: E-GEOD-35063 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress