Immunomagnetic purification of mES derived neuronal precursors increases neuronal migration and differentiation in vivo

ABSTRACT: All established protocols for differentiation of mouse and human pluripotent stem cells into specific neural subpopulations generate a considerable cellular heterogeneity that hampers experimental and clinical progress. In order to obtain a homogenous population of neuronal precursor cells and to streamline the differentiation of embryonic stem cells (ESCs), we assessed PSA-NCAM, a surface glycoprotein that is specifically expressed on immature neurons. We developed an optimized strategy for magnetic isolation of PSA-NCAM positive neuronal precursors from differentiated ESC cultures and characterized their neuronal differentiation potential in vitro. PSA-NCAM enrichment at an early step of neural differentiation increased the number of ES cell derived neurons and reduced cellular diversity

ORGANISM(S): Mus musculus

SUBMITTER: Stefan Tomiuk 

PROVIDER: E-GEOD-35125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress