Transcription profiling of E.coli ydgG mutant vs. isogenic wild type in a mature biofilm

ABSTRACT: The E. coli Genechip antisense genome array was used to study the differential gene expression profile of E. coli K12 ydgG mutant compared to its isogenic wild type in a mature biofilm. Experiment Overall Design: Overnight cultures were diluted 100X into 1 L shake flasks containing 250 mL of LB + 0.2 % and 10 g of glass wool. The cells were shaken at 250 rpm and 37 C for 24 hours (final OD600=5 for the suspended cells), to form a biofilm on the glass wool, and RNA was isolated from the biofilm and Affymetrix protocol was used for preparing fragmented and labelled cDNA that was hybrizide to the chips.

ORGANISM(S): Escherichia coli

SUBMITTER: Thomas Wood 

PROVIDER: E-GEOD-3514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress