Gene Expression Profiling Following DNA Vaccination of Rainbow Trout against Infectious Hematopoietic Necrosis Virus

ABSTRACT: The Infectious Hematopoietic Necrosic Virus (IHNV) DNA vaccine is based on the viral glycoprotein gene (G gene) and induces a non-specific anti-viral immune response and long-term specific immunity against IHNV in salmonid fishes. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine. Gene expression at the I.M. site was evaluated using a 16,000 feature salmon cDNA microarray. Eighty different transcripts were significantly modulated in the vector DNA group while 910 transcripts were modulated in the IHNV DNA vaccinated group relative to control group. Quantitative reverse transcriptase PCR was used to examine expression of selected genes at the

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Krista Nichols 

PROVIDER: E-GEOD-3522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress