A Gene Expression Profile in the Kidney After Cisplatin Treatment

ABSTRACT: Clinical use of a major cancer chemotherapeutic agent, cisplatin, is restricted to dose-limiting renal toxicity. A transcriptomic approach enabled us to extract putative molecules including Pleckstrin homology-like domain, family A, member-3 (Phlda3) contributing to the renal injury. This study investigated the expression of Phlda3 and its regulatory role in tubular injury induced by cisplatin in vivo and in vitro. Real-time PCR and immunoblot assays confirmed that Phlda3 was highly up-regulated in the kidney of mice after a single dose of cisplatin treatment, which preceded increases in the blood urea nitrogen or serum creatinine levels. Particularly, the level of Phlda3 transcript was substantially increased at an early time than that of kidney injury molecule-1, and remained elevated. T

ORGANISM(S): Mus musculus

SUBMITTER: Sang Geon Kim 

PROVIDER: E-GEOD-35257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress