Essential and unexpected role of YY1 to promote mesodermal cardiac differentiation

ABSTRACT: Rationale: Cardiogenesis is regulated by a complex interplay between transcription factors and chromatin-modifying enzymes. However, little is known about how these interactions regulate the transition from mesodermal precursors to cardiac progenitor cells (CPCs). Objective: To identify novel regulators of mesodermal cardiac lineage commitment. Methods and Results: We performed a bioinformatic-based transcription factor-binding site analysis on upstream promoter regions of genes that are enriched in ES cell-derived CPCs. From 32 candidate transcription factors screened, we found that YY1, a repressor of sarcomeric gene expression, is present in CPCs in vivo. Interestingly, we uncovered the ability of YY1 to transcriptionally activate Nkx2.5, a key marker of early cardiogenic commitment.

ORGANISM(S): Mus musculus

SUBMITTER: Serge Gregoire 

PROVIDER: E-GEOD-35370 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress