Epigenetic modifications and chromosome conformations of the beta globin locus throughout development

ABSTRACT: Human embryonic stem cells provide an alternative to using human embryos for studying developmentally regulated gene expression. The co-expression of high levels of embryonic epsilon and fetal gamma globin by the hESC-derived erythroblasts allows the interrogation of epsilon globin regulation at the transcriptional and epigenetic level which could only be attained previously by studying cell lines or transgenic mice. In this study, we compared the histone modifications across the beta globin locus of the undifferentiated hESCs and hESC-, FL-, and mobilized PB CD34+ cells-derived erythroblasts, which have distinct globin expression patterns corresponding to their developmental stages. We demonstrated that the histone codes employed by the beta globin locus are conserved throughout developme

ORGANISM(S): Homo sapiens

SUBMITTER: Kai-Hsin Chang 

PROVIDER: E-GEOD-35375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress