MicroRNA expression in normal and tumor breast tissues

ABSTRACT: microRNA expression signatures can differentiate normal and breast cancer tissues and can define specific clinico-pathological phenotypes in breast tumors. In order to further evaluate the microRNA expression profile in breast cancer, we analyzed the expression of 667 microRNAs in 29 tumors and 21 adjacent normal tissues using TaqMan Low-density arrays. 130 miRNAs showed significant differential expression (adjusted P value=0.05, Fold Change=2) in breast tumors compared to the normal adjacent tissue. Importantly, the role of 43 of these microRNAs has not been previously reported in breast cancer, including several evolutionary conserved microRNA*, showing similar expression rates to that of their corresponding leading strand. The procedure begins with the retro-transcription of 70ng of tot

ORGANISM(S): Homo sapiens

SUBMITTER: Alfredo Hidalgo-Miranda 

PROVIDER: E-GEOD-35412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress