Characterization of colon cancer cells: a functional approach characterizing CD133 as a potential stem cell marker

ABSTRACT: Background: Isolation and characterization of tumourigenic colon cancer initiating cells may help to develop novel diagnostic and therapeutic procedures. Methods: We characterized a panel of fourteen human colon carcinoma cell lines and their corresponding xenografts for the surface expression of different potential stem cell markers: CD133, CD24, CD44, CDCP1 and CXCR4. In five cell lines and nine xenografts mRNA expression of the investigated markers was determined. Tumour growth behaviour of CD133+, CD133- and unsorted SW620 cells was evaluated in vivo. Results: All surface markers showed distinct expression patterns in the examined tumours. Analyses of the corresponding xenografts revealed a significant reduction of cell numbers expressing the investigated markers. CD44 and CXCR4 mR

ORGANISM(S): Homo sapiens

SUBMITTER: Jaga Virayah 

PROVIDER: E-GEOD-35478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress